Development of a CRISPR/Cas12a-recombinase polymerase amplification assay for visual and highly specific identification of the Congo Basin and West African strains of mpox virusArticle Published on 2023-05-012023-06-10 Journal: Journal of medical virology [Category] 원숭이두창, [키워드] Cas12a clustered regularly interspaced short palindromic repeats Mpox virus recombinase polymerase amplification visualization. [DOI] 10.1002/jmv.28757 PMC 바로가기
Specific lateral flow detection of isothermal nucleic acid amplicons for accurate point-of-care testingArticle Published on 2023-02-152023-06-23 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 신종인플루엔자, [키워드] lateral flow assay peptide nucleic acids point-of-care testing recombinase polymerase amplification SARS-CoV-2. [DOI] 10.1016/j.bios.2022.114989 PMC 바로가기
Multiple ligation-Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2Article Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome amplification Analysis Bacterial colorimetric detection Colorimetric sensing conditions coronavirus detection system diagnosing disease Diseases effective Genome isothermal isothermal amplification limit of detection Multiple PK-probe point-of-care polymerase probe Pyrophosphate recombinase polymerase amplification Recombinase polymerase amplification (RPA) RNA robust SARS-CoV-2 SARS-CoV-2. selective sensitivity [DOI] 10.1016/j.talanta.2022.123835 PMC 바로가기
Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2Article Published on 2022-12-012022-11-15 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome amplification amplify catalytic clinical sample coronavirus coronavirus disease COVID-19 develop Diagnosis gold standard highlighting HPV-16 limit of detection Nanozyme strips nontoxic nucleic acid nucleic acid amplification Nucleic acid detection pandemic POCT point-of-care point-of-care testing polymerase REcombinase recombinase polymerase amplification reliability RPA RT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2. subsequent TMB unique viral infections virus [DOI] 10.1016/j.bios.2022.114739 PMC 바로가기
Automated, portable, and high-throughput fluorescence analyzer (APHF-analyzer) and lateral flow strip based on CRISPR/Cas13a for sensitive and visual detection of SARS-CoV-2SARS-CoV-2의 감도 및 시각적 검출을 위한 CRISPR/Cas13a 기반의 자동화된 휴대용 고처리량 형광 분석기(APHF 분석기) 및 측면 흐름 스트립Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addressed amplification automated biosensor Care Contamination coronavirus COVID-19 CRISPR CRISPR/Cas13a detect Detection limit develop globe Health Lateral lateral flow strip MOST On-site detection ORF1ab gene POCT Point point of care Point of care testing (POCT) polymerase provided recombinase polymerase amplification Recombinase polymerase amplification (RPA) RPA S gene SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2. severe acute respiratory syndrome Coronavirus specificity Visualization [DOI] 10.1016/j.talanta.2022.123594 PMC 바로가기 [Article Type] Article
Rational Design of CRISPR/Cas12a-RPA Based One-Pot COVID-19 Detection with Design of Experiments실험 계획을 통한 CRISPR/Cas12a-RPA 기반 One-Pot COVID-19 탐지의 합리적 설계Article Published on 2022-04-152022-09-11 Journal: ACS synthetic biology [Category] COVID19(2023년), SARS, 진단, 치료기술, [키워드] accelerate addition amplification applied approach benefit buffer complex component components condition COVID-19 COVID-19 pandemic CRISPR CRISPR/Cas12a critical impact definitive screening design demonstrated design of experiments detection Detection sensitivity diagnostic Diagnostic method diagnostics DNA DNA sequence DoE Effect effective experiment Factor full-length SARS-CoV-2 Genome highlight isothermal molecular Molecular diagnosis N gene one-pot COVID-19 testing optimization polymerase reaction reaction optimization Reactions recombinase polymerase amplification recombinase polymerase amplification (RPA). reverse transcription RNA RNase RNase inhibitor robust RPA RT-qPCR sensitivity significant effect significantly simple statistical variables [DOI] 10.1021/acssynbio.1c00617 PMC 바로가기 [Article Type] Article
A one-pot CRISPR/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics저렴하고 신속한 핵산 진단을 위한 원팟 CRISPR/Cas13a 기반 무오염 바이오센서Article Published on 2022-04-152022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, [키워드] 2019 coronavirus disease acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 African amplicon contamination amplification automated carried caused centrifugation Chain Reaction cleavage Complete completion Contamination Contamination-free detection coronavirus disease Coronavirus-2 COVID-19 CRISPR CRISPR/Cas13a diagnostic diagnostic testing diagnostics Diseases DNA virus effective Epidemic equipment Fever hydrophobic independent Infectious diseases Inner lid limit of detection Low-cost detection meet negative and positive nucleic acid Nucleic acid detection Nucleic acid diagnostics One-pot reaction. outbreak pandemic PCR polymerase chain polymerase chain reaction public health Quantitative raised Rapid REcombinase recombinase polymerase amplification reliability required RNA virus RPA SARS-CoV-2 shaking spreading Treatment tube vessel virus were used [DOI] 10.1016/j.bios.2022.113994 PMC 바로가기 [Article Type] Article
Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay outputSARS-CoV-2 감지를 위한 재조합 효소 중합효소 증폭 및 DNA 주형 반응을 이중 형광 및 측면 흐름 분석 출력과 결합Article Published on 2022-04-012022-09-11 Journal: Biopolymers [Category] COVID19(2023년), SARS, 치료기술, [키워드] absence acid acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification applied Biotin chain Chain Reaction coronavirus coumarin diagnostic disease detection DNA DNA templated reaction early disease early phase effective exacerbated Isolation lateral flow lateral flow assay magnitude methodology naked eye nucleic acid nucleic acid sensing. pandemic Patient peptide PNA point-of-care polymerase polymerase chain polymerase chain reaction reaction recombinase polymerase amplification RNA sequence RPA SARS-CoV-2 severe acute respiratory syndrome Coronavirus stimulated the SARS-CoV-2 transfer Transmission Vaccines while [DOI] 10.1002/bip.23485 PMC 바로가기 [Article Type] Article
Rapid and simultaneous visual screening of SARS-CoV-2 and influenza virufses with customized isothermal amplification integrated lateral flow stripArticle Published on 2021-11-062023-06-14 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), MERS, SARS, [키워드] Influenza virus lateral flow strip recombinase polymerase amplification SARS-CoV-2 Simultaneous identification [DOI] 10.1016/j.bios.2021.113771 PMC 바로가기 [Article Type] Article
An ultrafast SARS-CoV-2 virus enrichment and extraction method compatible with multiple modalities for RNA detectionRNA 검출을 위한 다중 양식과 호환되는 초고속 SARS-CoV-2 바이러스 농축 및 추출 방법Randomized Controlled Trial Published on 2021-10-022022-09-11 Journal: Analytica chimica acta [Category] MERS, SARS, 임상, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affinity Affinity nanoparticles alternative method alternative methods amplification Analysis Chain Reaction characterization characterized clinical samples clinically comparable compatibility Concordance coronavirus Coronavirus-2 COVID-19 pandemic demonstrated diagnoses Dilution dilutions double-blind study Enzymatic extraction form isothermal amplification Laboratory LAMP Loop-mediated amplification (LAMP) Loop-mediated isothermal amplification miniaturization nasopharyngeal swabs Particle pathogenicity polymerase polymerase chain polymerase chain reaction Polymerase chain reaction (PCR) positive recombinase polymerase amplification Recombinase polymerase amplification (RPA) respiratory reverse transcription reverse transcription real-time polymerase chain reaction RNA RNA eluate RNA isolation RNA virus RPA RT-PCR Saliva SARS-CoV-2 SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus-2 Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). solution Stage the stage transmission rate Viral viral titer viral titers viral transport media VTM zoonotic [DOI] 10.1016/j.aca.2021.338846 PMC 바로가기 [Article Type] Randomized Controlled Trial